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COMMENT ON NATIONAL STANDARD METHODS
KEY:
I
= Issued
UR / I
= Issued and Under Review
UR
= Under Review
D
= Draft
WD =
Withdrawn
UC =
Under Consultation
C = Contact Standards Unit
National Standard Methods - Bacteriology SOPs
Some National Standard Methods are available in Italian
|
Ref No |
Title |
Status |
Issue No |
Issue Date |
|
BSOP 1 |
Investigation of ear swabs and associated specimens |
I |
8.2 |
Oct 08 |
|
BSOP 10 |
Processing of faeces for Clostridium difficile |
I |
1.3 |
Sep 08 |
|
BSOP 11 |
Investigation of skin, superficial, and non-surgical wound swabs |
I |
5 |
Nov 09 |
|
GP Ref. Guide 11.rg |
N/A |
N/A |
N/A |
|
|
BSOP 14 |
Investigation of abscesses and deep-seated wound infections |
I |
5 |
Nov 09 |
|
BSOP 15 |
Investigation of bile |
I |
5 |
Apr 08 |
|
BSOP 17 |
Investigation of tissues and biopsies |
I |
5.1 |
Dec 09 |
|
BSOP 19 |
Investigation of sinus aspirate |
I |
7.1 |
Dec 09 |
|
BSOP 2 |
Investigation of eye swabs and canalicular pus |
I |
5 |
Mar 08 |
|
BSOP 20 |
Investigation of intravascular cannulae and associated specimens |
I |
5 |
May 08 |
|
BSOP 22 |
Investigation of cerebrospinal fluid shunts |
I |
5 |
Nov 09 |
|
BSOP 23 |
Investigation of gastric aspirates and infection screen swabs from neonates |
I |
4.1 |
May 05 |
|
BSOP 23.dd |
Investigation of gastric aspirates and infection screen swabs from neonates |
UR |
N/A |
N/A |
|
BSOP 25 |
Investigation of continuous ambulatory peritoneal dialysis fluid |
I |
5.1 |
Dec 09 |
|
BSOP 26 |
Investigation of fluids from normally sterile sites |
I |
5 |
Nov 09 |
|
BSOP 27 |
Investigation of cerebrospinal fluid |
I |
5 |
Apr 08 |
|
BSOP 28 |
Investigation of genital tract and associated specimens |
I |
4.1 |
May 05 |
|
BSOP 28.dm |
Investigation of genital tract and associated specimens |
UC |
N/A |
N/A |
|
GP Ref. Guide 28.rg |
Management of Abnormal Vaginal Discharge in women - Quick Reference Guide for Primary Care |
N/A |
N/A |
N/A |
|
BSOP 29 |
Investigation of specimens for screening for MRSA |
I |
5.1 |
Oct 08 |
|
GP Ref. Guide 29.rg |
MRSA Screening and Suppression - Quick Reference Guide for Primary Care |
N/A |
N/A |
N/A |
|
BSOP 30 |
Investigation of faecal specimens for bacterial pathogens |
I |
7 |
Sep 10 |
|
GP Ref. Guide 30.rg |
N/A |
N/A |
N/A |
|
|
BSOP 31 |
Investigation of specimens other than blood for parasites |
I |
3 |
Jul 08 |
|
BSOP 36 |
Investigation of Specimens For Ectoparasites PLEASE SEE QSOP 67 |
N/A |
N/A |
N/A |
|
BSOP 37 |
Investigation of blood cultures (for organisms other than Mycobacterium species) |
I |
6 |
Jul 10 |
|
BSOP 38 |
Investigation of Bone Marrow |
I |
1 |
Nov 07 |
|
BSOP 39 |
Investigation of dermatological specimens for superficial mycoses |
I |
2 |
Sep 10 |
|
GP Ref. Guide 39.rg |
N/A |
N/A |
N/A |
|
|
BSOP 4 |
Investigation of mouth swabs |
I |
6.1 |
Oct 08 |
|
BSOP 40 |
Investigation of specimens for Mycobacterium species |
I |
5 |
Jul 06 |
|
BSOP 40.dk |
Investigation of specimens for Mycobacterium species |
UR |
N/A |
N/A |
|
BSOP 41 |
Investigation of Urine |
I |
7 |
Oct 09 |
|
GP Ref. Guide 41.rg |
N/A |
N/A |
N/A |
|
|
BSOP 42 |
Investigation of bone and soft tissue associated with osteomyelitis |
I |
1.1 |
Dec 09 |
|
BSOP 44 |
Investigation of Prosthetic Joint Infection samples |
I |
1.1 |
Dec 09 |
|
BSOP 45 |
Susceptibility Testing PLEASE SEE QSOP 51 |
N/A |
N/A |
N/A |
|
BSOP 47 |
Investigation of specimens for Legionella species |
I |
5 |
Nov 09 |
|
BSOP 5 |
Investigation of nose swabs |
I |
6.1 |
Oct 08 |
|
BSOP 51 |
Screening for Meningococci |
I |
1.1 |
May 05 |
|
BSOP 51.da |
Screening for Meningococci |
UR |
N/A |
N/A |
|
BSOP 52 |
Investigation of intraocular fluids and corneal scrapings |
I |
5 |
Apr 08 |
|
BSOP 55 |
Investigation of gastric biopsies for Helicobacter pylori |
I |
5 |
Aug 08 |
|
GP Ref. Guide 55.rg |
Diagnosis of Helicobacter pylori (HP) in Dyspepsia - Quick Reference Guide for Primary Care |
N/A |
N/A |
N/A |
|
BSOP 57 |
Investigation of Bronchoalveolar Lavage, Sputum and Associated Specimens |
I |
2.3 |
Dec 09 |
|
BSOP 58 |
Processing Swabs For Group B Streptococcal Carriage |
I |
2.1 |
Jun 06 |
|
BSOP 58.da |
Processing Swabs For Group B Streptococcal Carriage |
UR |
N/A |
N/A |
|
BSOP 6 |
Investigation of specimens for Bordetella pertussis and Bordetella parapertussis |
I |
7 |
Nov 08 |
|
BSOP 9 |
Investigation of throat swabs |
I |
8 |
Dec 09 |
Number of Issued Bacteriology SOPs: 35
National Standard Methods - Bacteriology Identification
|
Ref No |
Title |
Status |
Issue No |
Issue Date |
|
BSOPID 1 |
Introduction to the preliminary identification of medically important bacteria |
I |
1.4 |
Feb 08 |
|
BSOPID 10 |
Identification of aerobic Actinomycetes |
I |
1 |
Nov 09 |
|
BSOPID 11 |
Identification of Moraxella Species and Morphologically Similar Organisms |
I |
2.1 |
Jul 11 |
|
BSOPID 12 |
Identification of Haemophilus species and the HACEK group of organisms |
I |
2 |
Apr 09 |
|
BSOPID 13 |
Identification of Pasteurella species and morphologically similar bacteria |
I |
2.1 |
Sep 07 |
|
BSOPID 14 |
Identification of anaerobic cocci |
I |
2 |
Dec 09 |
|
BSOPID 15 |
Identification of anaerobic Actinomycetes species |
I |
1 |
Dec 09 |
|
BSOPID 16 |
Identification of Enterobacteriaceae |
I |
3 |
Sept 10 |
|
BSOPID 17 |
Identification of glucose non-fermenting Gram-negative rods |
I |
2 |
Apr 07 |
|
BSOPID 18 |
Identification of Legionella species |
I |
2 |
Nov 07 |
|
BSOPID 19 |
Identification of Vibrio species |
I |
2 |
Nov 07 |
|
BSOPID 2 |
Identification of Corynebacterium species |
I |
3 |
Jul 08 |
|
BSOPID 20 |
Identification of Shigella species |
I |
2 |
Nov 07 |
|
BSOPID 21 |
Identification of Yersinia species from faeces |
I |
2 |
Nov 07 |
|
BSOPID 22 |
Identification of Escherichia coli O157 |
I |
3 |
Sept 10 |
|
BSOPID 23 |
Identification of Campylobacter species |
I |
2 |
Nov 07 |
|
BSOPID 24 |
Identification of Salmonella species |
I |
2.1 |
Sep 08 |
|
BSOPID 25 |
Identification of anaerobic Gram-negative rods |
I |
1 |
Dec 09 |
|
BSOPID 26 |
Identification of Helicobacter species |
I |
2 |
Feb 08 |
|
BSOPID 3 |
Identification of Listeria species, non-toxigenic Corynebacterium species and other non-sporing gram-positive rods |
I |
2 |
Oct 07 |
|
BSOPID 4 |
Identification of Streptococcus species, Enterococcus species and morphologically similar organisms |
I |
2.1 |
Sep 07 |
|
BSOPID 5 |
Identification of Bordetella pertussis and Bordetella parapertussis from selective agar |
I |
2.1 |
Sep 07 |
|
BSOPID 6 |
Identification of Neisseria species |
I |
2.1 |
Sep 07 |
|
BSOPID 6.db |
Identification of Neisseria species |
UR |
N/A |
N/A |
|
BSOPID 7 |
Identification of Staphylococcus species, Micrococcus species and Rothia species |
I |
2.1 |
Sep 07 |
|
BSOPID 8 |
Identification of Clostridium species |
I |
3 |
Jul 08 |
|
BSOPID 9 |
Identification of Bacillus species |
I |
2.1 |
Sep 07 |
Number of Issued Bacteriology ID SOPs: 26
National Standard Methods - Food
These documents have been removed and enquiries should be directed to Dr Jim McLauchlin at
National Standard Methods - Guidance Notes
|
Ref No |
Title |
Status |
Issue No |
Issue Date |
|
|
QSOP 1.df
|
Best practice approach on internal quality assurance |
D
|
N/A
|
N/A
|
|
|
QSOP 10
|
Traceability and use of thermometers in UKAS accredited laboratories |
WD
|
N/A
|
N/A
|
|
|
QSOP 13
|
Auditing in Health Protection Agency laboratories |
WD
|
N/A
|
N/A
|
|
|
QSOP 2
|
Local document control of documents issued by Technical Services. The Health Protection Agency |
WD
|
N/A
|
N/A
|
|
|
QSOP 23
|
Commercial and in-house diagnostic tests: Evaluations and Validations |
I
|
4.1
|
Sep 08
|
|
|
QSOP 23.da
|
Commercial and in-house diagnostic tests: Evaluations and Validations |
UR
|
N/A
|
N/A
|
|
|
QSOP 27
|
Quality assurance in the diagnostic virology and serology laboratory |
I
|
6
|
Nov 10
|
|
|
QSOP 30
|
Laboratory diagnosis of legionella infections in the HPA |
WD
|
N/A
|
N/A
|
|
|
QSOP 31
|
Surveillance of polio in the UK |
I
|
3.3
|
Nov 05
|
|
|
QSOP 31.de
|
Surveillance of polio in the UK |
UR
|
N/A
|
N/A
|
|
|
QSOP 33
|
European Directive on in vitro diagnostic medical devices (98/79/EC) |
I
|
4.1
|
Oct 08
|
|
|
QSOP 36
|
Corrective and preventive action |
WD
|
N/A
|
N/A
|
|
|
QSOP 37
|
Procedure for dealing with presumptive pathogens isolated from ready-to-eat foods |
WD
|
N/A
|
N/A
|
|
|
QSOP 38
|
Good laboratory practice when performing molecular amplification assays |
I
|
4
|
Sep 10
|
|
|
QSOP 4
|
Uncertainty of Measurement in Testing |
I
|
5
|
Aug 05
|
|
|
QSOP 42
|
Microbiological examination of CSFs, that may contain agents of spongiform encephalopathies |
I
|
2.2
|
Sep 05
|
|
|
QSOP 42.da
|
Microbiological examination of CSFs, that may contain agents of spongiform encephalopathies |
UR
|
N/A
|
N/A
|
|
|
QSOP 44
|
Microbiological investigation of patients with acute lymphadenopathy and fever |
I
|
2
|
Dec 08
|
|
|
QSOP 44.df
|
Microbiological investigation of patients with acute lymphadenopathy and fever |
UC
|
N/A
|
N/A
|
|
|
QSOP 46
|
Aminoglycoside and glycopeptide assays in clinical microbiology laboratories |
WD
|
N/A
|
N/A
|
|
|
QSOP 47
|
Chlamydial Zoonotic Infections |
I
|
1
|
Apr 09
|
|
|
QSOP 48
|
Investigation of Viral Encephalitis and Meningitis |
I
|
2.1
|
Aug 08
|
|
|
QSOP 48.dh
|
Investigation of Viral Encephalitis and Meningitis |
UR
|
N/A
|
N/A
|
|
|
QSOP 5
|
Handling and transport of samples (for the purposes of UKAS accreditation) |
WD
|
N/A
|
N/A
|
|
|
QSOP 51
|
Laboratory detection and reporting of bacteria with extended spectrum ß-Lactamases |
I
|
2.2
|
May 08
|
|
|
QSOP 51.da
|
Laboratory detection and reporting of bacteria with extended spectrum ß-Lactamases |
UR
|
N/A
|
N/A
|
|
|
QSOP 52
|
Inoculation of culture media (formerly BSOP 54) |
I
|
1
|
Feb 08
|
|
|
QSOP 53
|
Recommendations for the screening of specimens for Corynebacterium species |
I
|
2.1
|
Sep 08
|
|
|
QSOP 54
|
Investigation of Hepatitis |
I
|
1
|
Nov 10
|
|
|
QSOP 55
|
Investigation of Vesicular Rashes |
I
|
2
|
Jul 09
|
|
|
QSOP 55.df
|
Investigation of Vesicular Rashes |
UC
|
N/A
|
N/A
|
|
|
QSOP 56
|
Investigation of Red Rash |
|
N/A
|
N/A
|
|
|
QSOP 58
|
Presumptive diagnosis of Cronobacter sakazakii infection of infants |
I
|
2
|
Mar 10
|
|
|
QSOP 59
|
Investigation of Toxoplasma Infection in pregnancy |
I
|
2
|
Mar 10
|
|
|
QSOP 60
|
Respiratory viruses |
I
|
1
|
Aug 08
|
|
|
QSOP 62
|
Detection of Neisseria gonorrhoeae using Molecular methods |
I
|
1
|
Feb 10
|
|
|
QSOP 63
|
Assessment of point of care tests in Microbiology |
WD
|
N/A
|
N/A
|
|
|
QSOP 66
|
Neonatal Hepatitis Syndrome |
D
|
N/A
|
N/A
|
|
|
QSOP 67
|
Investigation of specimens for ectoparasites |
I
|
1
|
Nov 10
|
Number of Issued Guidance Notes SOPs: 20
Food related documents have been removed and enquiries should be directed to Dr Jim McLauchlin at
National Standard Methods - Test Procedures
|
Ref No |
Title |
Status |
Issue No |
Issue Date |
|
BSOPTP 1 |
Example reference strains for National Standard Method Test Procedures |
I |
1 |
Dec 10 |
|
BSOPTP 10 |
Coagulase test |
I |
4.1 |
Dec 10 |
|
BSOPTP 12 |
Deoxyribonuclease test |
I |
2.2 |
Jul 11 |
|
BSOPTP 19 |
Indole test |
I |
2.1 |
Dec 10 |
|
BSOPTP 2 |
Aesculin hydrolysis test |
I |
2.1 |
Dec 10 |
|
BSOPTP 21 |
Motility test |
I |
2.1 |
Dec 10 |
|
BSOPTP 22 |
Nagler test |
I |
2.1 |
Dec 10 |
|
BSOPTP 24 |
ONPG (ß-galactosidase) test |
I |
2.1 |
Dec 10 |
|
BSOPTP 25 |
Optochin test |
I |
2.1 |
Dec 10 |
|
BSOPTP 26 |
Oxidase test |
I |
2.1 |
Dec 10 |
|
BSOPTP 27 |
Oxidation and fermentation of glucose test |
I |
2.1 |
Dec 10 |
|
BSOPTP 29 |
Porphyrin synthesis (ala) test |
I |
2.1 |
Dec 10 |
|
BSOPTP 3 |
Agglutination test |
I |
2 |
Mar 10 |
|
BSOPTP 30 |
Potassium hydroxide test |
I |
2 |
Dec 10 |
|
BSOPTP 32 |
Changing the phase of Salmonella |
I |
2 |
May 10 |
|
BSOPTP 34 |
Thermonuclease test |
I |
2 |
Dec 10 |
|
BSOPTP 36 |
Urease test |
I |
2 |
Dec 10 |
|
BSOPTP 38 |
X and V factor test |
I |
2.3 |
Jan 11 |
|
BSOPTP 39 |
Staining Procedures |
I |
1 |
Mar 07 |
|
BSOPTP 5 |
Bile solubility test |
I |
2.1 |
Jul 10 |
|
BSOPTP 8 |
Catalase test |
I |
2.1 |
Dec 10 |
Number of Issued Test Procedures SOPs: 21
National Standard Methods - Milk and Dairy
These documents have been removed and enquiries should be directed to Dr Jim McLauchlin at
National Standard Methods - Virology
|
Ref No |
Title |
Status |
Issue No |
Issue Date |
|
VSOP 10 |
Blood borne virus testing in dialysis patients |
I |
1 |
Feb 08 |
|
VSOP 11 |
Anti-HIV Screening |
I |
2 |
Aug 09 |
|
VSOP 11.df |
Anti-HIV Screening |
UC |
N/A |
N/A |
|
VSOP 12 |
Preparation of coated grids for electron microscopy |
I |
6 |
Mar 10 |
|
VSOP 13 |
Electron Microscopy: Flotation (direct) method |
I |
6 |
Jun 10 |
|
VSOP 14 |
Electron Microscopy: Differential Centrifugation (double-spin method) |
I |
6 |
Jun 10 |
|
VSOP 15 |
Electron microscopy: ammonium sulphate precipitation |
I |
5 |
Sep 07 |
|
VSOP 16 |
Electron microscopy using solid phase immune electron microscopy |
I |
5 |
Sep 07 |
|
VSOP 17 |
Isolation of herpes simplex virus associated with herpes genitalis |
I |
4 |
Jul 07 |
|
VSOP 17.da |
Isolation of herpes simplex virus associated with herpes genitalis |
UR |
N/A |
N/A |
|
VSOP 18 |
Complement fixation tests |
I |
3 |
Dec 09 |
|
VSOP 2 |
Gastroenteritis: sporadic cases |
I |
5 |
Jul 07 |
|
VSOP 20 |
WD |
N/A |
N/A |
|
|
VSOP 21 |
Isolation of Viruses associated with infections of the eye: Keratoconjunctivitis |
I |
3 |
Jun 10 |
|
VSOP 22 |
Immunofluorescence and isolation of viruses from respiratory samples |
I |
1 |
Jul 07 |
|
VSOP 23 |
Isolation of human herpes viruses (excluding herpes genitalis) |
I |
3 |
Nov 09 |
|
VSOP 24 |
Isolation of enteroviruses and parechoviruses |
I |
3 |
Nov 09 |
|
VSOP 25 |
Real-Time Quadriplex PCR for the detection of Influenza |
I |
1 |
Nov 06 |
|
VSOP 26 |
Epstein-Barr Virus serology (formerly VSOP 6.2) |
I |
3 |
Mar 08 |
|
VSOP 27 |
Hepatitis A virus acute infection serology (formerly VSOP6.3) |
I |
2.4 |
Oct 07 |
|
VSOP 28 |
Cytomegalovirus serology (formerly VSOP6.4) |
I |
2.4 |
Oct 07 |
|
VSOP 29 |
C |
2 |
May 09 |
|
|
VSOP 3 |
Gastroenteritis: Outbreaks |
I |
6 |
Aug 07 |
|
VSOP 30 |
Investigation of pregnant women exposed to rash illnesses (now incorporating VSOPs 31, 32, 33 and 34) |
I |
4 |
May 09 |
|
VSOP 31 |
WD |
N/A |
N/A |
|
|
VSOP 32 |
WD |
N/A |
N/A |
|
|
VSOP 33 |
Pregnant patient in contact with rash illness (now incorporated in VSOP 30) |
WD |
N/A |
N/A |
|
VSOP 34 |
Investigation of pregnant patient with rash illness (now incorporated in VSOP 30) |
WD |
N/A |
N/A |
|
VSOP 35 |
Investigation of Cytomegalovirus infection by Roche Lightcycler PCR |
I |
2.1 |
Aug 07 |
|
VSOP 36 |
Operation of the Roche MagNA Pure LC automated nucleic acid extraction robot |
WD |
N/A |
N/A |
|
VSOP 37 |
Chlamydia trachomatis Infection - Testing by Nucleic Acid Amplification Test (NAATs) |
I |
2.1 |
Sep 08 |
|
VSOP 37.df |
Chlamydia trachomatis Infection - Testing by Nucleic Acid Amplification Test (NAATs) |
UC |
N/A |
N/A |
|
GP Ref. Guide 37.rg |
Diagnosis of Chlamydia - Quick Reference Guide for General Practices |
N/A |
N/A |
N/A |
|
VSOP 38 |
Detection of SARS-CoV by reverse transcription PCR |
I |
2 |
May 09 |
|
VSOP 39 |
Procedure for the care and propagation of cell cultures for virus isolation |
I |
2 |
Dec 09 |
|
VSOP 4 |
Hepatitis B diagnostic serology in the immunocompetent (including Hepatitis B in pregnancy) |
I |
5 |
Nov 10 |
|
VSOP 40 |
RT-PCR (Block Based) for Influenza H5 viruses |
I |
4 |
May 09 |
|
VSOP 41 |
Real-Time RT-PCR (Taq Man) for influenza A H5 viruses |
I |
5 |
May 09 |
|
VSOP 42 |
RT-PCR for the detection of influenza A viruses |
I |
2 |
May 09 |
|
VSOP 43 |
Investigation of viral encephalitis |
I |
2.1 |
Apr 11 |
|
VSOP 44 |
Serological Diagnosis of Syphilis |
I |
1.1 |
Jul 11 |
|
VSOP 45 |
Haemadsorption of viruses |
I |
2 |
Mar 10 |
|
VSOP 46 |
European Real-Time RT-PCR for Influenza A H5 viruses |
I |
2 |
May 09 |
|
VSOP 47 |
Investigation of Swine-Lineage Influenza A (H1N1) by PCR |
I |
3 |
Jun 09 |
|
VSOP 48 |
Swine-lineage Influenza A N1 Fast Real Time Confirmatory PCR assay |
C |
1 |
May 09 |
|
VSOP 49 |
Investigation of Swine-Lineage Influenza A/H1N1 v using the Quadriplex Influenza A PCR assay with Swine-Lineage Influenza A H1 and N1 assays |
I |
1.1 |
Jun 09 |
|
VSOP 5 |
Investigation of Hepatitis C Infection infection by antibody testing or combined antigen/antibody assay |
I |
6 |
Nov 10 |
|
VSOP 50 |
C |
1 |
Jun 09 |
|
|
VSOP 51 |
Investigation of Swine-lineage Influenza A/H1N1v using the Swine-lineage Influenza A H1 assay |
I |
1 |
Jul 09 |
|
VSOP 52 |
Pandemic Influenza A H1N1 2009 H275Y (Oseltamivir Resistance) Screening Assay (TaqMan PCR) |
C |
2 |
Jan 11 |
|
VSOP 6 |
WD |
N/A |
N/A |
|
|
VSOP 8 |
Vertical HCV transmission algorithm |
I |
1 |
Nov 07 |
Number of Issued Virology SOPs: 37
National Standard Methods - Water
These documents have been removed and enquiries should be directed to Dr Jim McLauchlin at
National Standard Methods - Syndromic Algorithms
|
Ref No |
Title |
Status |
Issue No |
Issue Date |
|
S1 |
Acute Infective Hepatitis |
I |
1 |
Feb 10 |
|
S2 |
Pneumonia |
I |
1 |
Jul 11 |
|
S3 |
Conjunctivitis |
I |
1 |
Feb 10 |
|
S6.dp |
Sexually Transmitted Infections |
UC |
N/A |
N/A |
Number of SOPs: 3